Proteins found in the membranes of cells facilitate communication between the cell and its environment. One of the main means of communication is by the movement of ions, such as calcium, sodium, and potassium, through channels formed by membrane proteins. We investigate the assembly and function of channel forming membrane proteins with the use of electrochemical techniques, such as impedance spectroscopy, and fluorescence. We also utilize a planar, polymer supported lipid bilayer platform that mimics the cellular membrane environment; proteins may then be integrated into the lipid bilayer in an ordered manner. This lipid bilayer platform has been optimized to allow proteins to sit in their natural conformation and for maximal resistance to ion movement across the bilayer, thus directing all ions to pass through any open protein channels. This work serves to elucidate the behavior and selectivity of protein channels that may play significant roles in cell trafficking and communication.